Metal ion transport quantified by ICP-MS in intact cells
نویسندگان
چکیده
The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red blood cells. The ICP-MS method provides greater precision and statistical power than possible with conventional tracer flux methods. The method is widely applicable to studies of other metal ion transporters and metal-dependent processes in a range of cell types and conditions.
منابع مشابه
Polysulfone-based Anion Exchange Membranes for Potential Application in Solid Alkaline Fuel Cells
In present research work, anion exchange membranes based on quaternized polysulfone with ammonium cation moieties (QAPSF) were prepared by chloromethylation, amination and alkalization. The chloromethylated polysulfone were characterized by 1HNMR spectroscopy and functionalization degree was determined according to peak area integration. Ion transport properties (ionic conductivity, ion exchang...
متن کاملAn Introduction to Single Cell ICP - MS Analysis
The introduction and development of Single Particle ICP-MS (SP-ICP-MS) has opened a new area of research which allows the rapid detection and analysis of metal-based particles in a variety of matrices and applications.1-6 The key feature of SP-ICP-MS is that it allows discrete pulses of positively charged ions to be detected and measured in a time-resolved manner using microsecond (μs) data acq...
متن کاملEvaluation of the method for analyzing chromium, cobalt and titanium ion levels in the blood following hip replacement with a metal-on-metal prosthesis.
High-resolution inductively coupled plasma mass spectrometry (HR-ICP-MS) can be used to measure metal ion levels in whole blood, although the accuracy and repeatability of this method is not yet known in the clinical setting. In this study, chromium, cobalt and titanium ion levels were measured in three whole blood samples, each collected at the same moment from 101 patients undergoing total hi...
متن کاملElectrochemistry combined on-line with atomic mass spectrometry and related techniques for trace-metal analysis and electrode-reaction studies
Electrochemistry (EC) combined on-line with atomic mass spectrometry (MS) and related techniques (e.g., inductively coupled plasma atomic emission spectrometry, ICP–AES) affords an avenue for analysis of metals present at ultratrace levels and the effective elimination of matrix effects detrimental to atomic MS and spectrometric techniques. In addition, electrode reactions involving inorganic s...
متن کاملOn-line coupling of continuous-flow gel electrophoresis with inductively coupled plasma-mass spectrometry to quantitatively evaluate intracellular metal binding properties of metallochaperones HpHypA and HpHspA in E. coli cells.
On-line coupling of gel electrophoresis with inductively coupled plasma-mass spectrometry (GE-ICP-MS) offers a strategy to monitor intracellular metals and their associated proteins simultaneously. Herein, we examine the feasibility of the GE-ICP-MS system in the quantitative analysis of intracellular metal binding properties using two Helicobacter pylori metallochaperones HypA and HspA overexp...
متن کامل